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Use of reversed-phase HPLC for the purification of 5-phosphorylated oligonucleotides
Summary
Type:
HPLC
Application #:
107260
Detection:
UV, 254nm
Conditions:
eluent: gradient of methanol in 0.01M triethylammonium acetate from 10 to 40% in 30min flow rate 1.5ml/min
Authors:
Delort AM
Source:
J Chromatogr 283 (1984) 462 - 467
Details
Legend: Fig. 2. Separation of dA3TA8 (B) and its phosphorylated derivative (A).
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